References

smjournal

Спортивная медицина: наука и практика

Sports medicine: research and practice

2223-25242587-9014

NEICON

10.47529/2223-2524.2021.3.8

smjournal-307

Research Article

ФИЗИОЛОГИЯ И БИОХИМИЯ СПОРТА

SPORTS PHYSIOLOGY AND BIOCHEMISTRY

Перспективные направления лабораторной диагностики в спорте и фитнесе

Promising directions of laboratory diagnostics in spores and fitness

https://orcid.org/0000-0002-7904-9565

Загородный

Г. М.

Zaharodny

H. M.

Загородный Геннадий Михайлович, к.м.н., доцент, врач высшей категории по спортивной медицине, ведущий научный сотрудник

220020, Минск, Нарочанская ул., 8.

Hennady M. Zaharodny, M.D., Ph.D. (Medicine), Associate Professor, Doctor of the Highest Category in Sports Medicine, Leading Researcher

8, Narochanskaya str., Minsk, 220020

6911061@tut.by

Шераш

Н. В.

Sherash

N. V.

Шераш Наталья Владимировна, заведующая лабораторией биохимии спорта

220020, Минск, Нарочанская ул., 8

Natalya V. Sherash, Head of the Laboratory of Sports Biochemistry

8, Narochanskaya str., Minsk, 220020

Будко

А. Н.

Budko

A. N.

Будко Анастасия Николаевна, старший научный сотрудник лаборатории биохимии спорта

220020, Минск, Нарочанская ул., 8

Anastasia N. Budko, Senior Researcher of the Laboratory of Sports Biochemistry

8, Narochanskaya str., Minsk, 220020

Шведова

Н. В.

Shvedova

N. V.

Шведова Наталья Викторовна, научный сотрудник лаборатории биохимии спорта

220020, Минск, Нарочанская ул., 8

Natalya V. Shvedova, Researcher of the Laboratory of Sports Biochemistry

8, Narochanskaya str., Minsk, 220020

ГУ «Республиканский научно-практический центр спорта»БеларусьState University “Republican Scientific and Practical Center of Sports”Belarus

2021

13122021

1131827

2021

Загородный Г.М., Шераш Н.В., Будко А.Н., Шведова Н.В.

Zaharodny H.M., Sherash N.V., Budko A.N., Shvedova N.V.

Данная работа распространяется под лицензией Creative Commons Attribution 4.0.

This work is licensed under a Creative Commons Attribution 4.0 License.

https://www.smjournal.ru/jour/article/view/307

В статье изложены основные биомаркеры определения адаптации человека к физическим нагрузкам. Проанализированы современные научные публикации по критериям индивидуальной переносимости пищевых продуктов, изучены перспективные направления персонифицированной коррекции диеты. Авторами сформирована группа валидных (спорт-специфических) лабораторных показателей, предложено обращать пристальное внимание на референсные значения лабораторного оборудования, имеющего «свои» нормы. Перспективным направлением лабораторно-диагностической работы является формирование достоверных и доступных взаимодополняющих критериев, находящихся на стыке функциональных и инструментальных методов диагностики. Метаболомика рассматривается как молодая, но весьма эффективная для обнаружения метаболических высокоспецифичных характеристик состояния здоровья человека наука. Авторами предложены основные направления научных исследований в лабораторной диагностике в спорте.

The article describes the main biomarkers for determining human adaptation to physical activity. Analyzed modern scientific publications on the criteria of individual tolerance of food products, studied promising directions of personalized correction of the diet. The authors have formed a group of valid (sports-specific) laboratory indicators, it is proposed to pay close attention to the reference values of laboratory equipment that have “their own” norms. A promising direction of laboratory diagnostic work is the formation of reliable and accessible complementary criteria that are at the “junction” of functional and instrumental diagnostic methods. Metabolomics is viewed as a young but highly effective science for detecting highly specific metabolic characteristics of human health. The authors proposed the main directions of scientific research in laboratory diagnostics in sports.

лабораторная диагностикаспортметаболомикапитаниеадаптациядиетафитнес

laboratory diagnosticssportsmetabolomicsnutritionadaptationdietfitness

References1

Занковец В.Э., Загородный Г.М. Анализ и оценка эффективности тренировочного процесса команды высокого спортивного мастерства. Прикладная спортивная наука. 2020;(1(11)):12–24.

Zankovets, V.E., Zaharodny G.M. Analysis and assessment of the effectiveness of the training process of a team of high sportsmanship. Prikladnaya sportivnaya nauka [Applied sports science]. 2020;(1(11)):12–24 (In Russ).

Ландырь А.П., Ачкасов Е.Е., Медведев И.Б. Тесты с дозируемой физической нагрузкой в спортивной медицине. Москва: ООО Издательство «Спорт»; 2019. 256 с.

Landyr A.P., Achkasov E.E., Medvedev I.B. Tests with dosed physical activity in sports medicine. Moscow: Sport Publ.; 2019. 256 p. (In Russ.).

Гаврилова Е.А. Клинические и экспертные вопросы электрокардиографии в спортивной медицине. СанктПетербург: Спорт; 2019. 273 с.

Gavrilova E.A. Clinical and expert issues of electrocardiography in sports medicine. St. Petersburg: Sport Publ.; 2019. 273 p. (In Russ.).

Платонов В.Н. Структура и содержание непосредственной подготовки спортсменов высокой квалификации к главным соревнованиям. Наука в олимпийском спорте. 2018;(2):17–41.

Platonov V.N. The structure and content of direct training of highly qualified athletes for the main competitions. Nauka v olimpiiskom sporte = Science in Olympic sports. 2018;(2):17–41 (In Russ.).

Курашвили В.А., Парастаев С.А., Поляев Б.А. Синдром переутомления: предикторы и биомаркеры (обзор зарубежных публикаций). B: XV международная научная конференция по вопросам состояния и перспективам развития медицины в спорте высших достижений «Спортмед-2020»: сборник материалов тезисов «СпортМед–2020» (ФМБА России); Москва, 10–11 декабря 2020 г.; Москва: РАСМиРБИ; 2020, с. 28–29.

Kurashvili V.A., Parastaev S.A., Polyaev B.A. Overfatigue syndrome: predictors and biomarkers (a review of foreign publications). In: XV international scientific conference on the state and prospects for the development of medicine in elite sports “Sportmed-2020”: Collection of abstracts “SportMed-2020” (FMBA of Russia); Moscow, 10-11.12.2020; Moscow: RASMiRBI; 2020. P. 28–29 (In Russ.).

Будко А.Н., Шераш Н.В., Гаврилова С.О. Сравнительный анализ изменений индивидуальных биохимических показателей у мужчин в шорт-треке в годичном цикле подготовки. Прикладная спортивная наука. 2020;(2(12)):33-43.

Budko A.N., Sherash N.V., Gavrilova S.O. Comparative analysis of changes in individual biochemical parameters in men in short track speed skating in the annual training cycle. Prikladnaya sportivnaya nauka [Applied sports science]. –2020;(2(12)):33–43 (In Russ.).

Ширковец Е.А., Рыбина И.Л. Комплексный анализ метаболитов энергообеспечения и эргометрических данных при стандартном тестировании пловцов высокой квалификации. Вестник спортивной науки. 2019;(1):61–66.

Shirkovets E.A., Rybina I.L. Complex analysis of metabolites of energy supply and ergometric data during standard testing of highly qualified swimmers. Vestnik sportivnoi nauki = Sports Science Bulletin. 2019;(1):61–66 (In Russ.).

Astolfi T., von Roten F.C., Kayser B., Saugy M., Faiss R. The Influence of Training Load on Hematological Athlete Biological Passport Variables in Elite Cyclists. Front. Sports Act. Living. 2021;3:618285. https://doi.org/10.3389/fspor.2021.618285

Simmons R., Doma K., Sinclair W.,Connor J., Leicht A. Acute Effects of Training Loads on Muscle Damage Markers and Performance in Semi-elite and Elite Athletes: A Systematic Review and Meta-analysis. Sports Med. 2021;51(10):2181–2207. https://doi.org/10.1007/s40279-021-01486-x

Hendrix J., Nijs J, Ickmans K., Godderis L., Ghosh M., Polli A. The Interplay between Oxidative Stress, Exercise, and Pain in Health and Disease: Potential Role of Autonomic Regulation and Epigenetic Mechanisms. Antioxidants (Basel). 2020;9(11):1166. https://doi.org/10.3390/antiox9111166

Biljak V.R., Vidranski V., Ružić L. Women in sports: the applicability of common national reference intervals for inflammatory and coagulation biomarkers. Biochem. Med (Zagreb). 2021;31(1):010702. https://doi.org/10.11613/BM.2021.010702

Reichel T., Boßlau T.K., Palmowski J.,Eder K., Ringseis R., Mooren F.C., et al. Reliability and suitability of physiological exercise response and recovery markers. Sci Rep. 2020;10(1):11924. https://doi.org/10.1038/s41598-020-69280-9

Varamenti E., Tod D., Pullinger S.A. Redox Homeostasis and Inflammation Responses to Training in Adolescent Athletes: a Systematic Review. Sports Med. Open. 2020;6(1):34. https://doi.org/10.1186/s40798-020-00262-x

Płoszczyca K., Jazic D., Piotrowicz Z., Chalimoniuk M. Comparison of maximal lactate steady state with anaerobic threshold determined by various methods based on graded exercise test with 3-minute stages in elite cyclists. BMC Sports Sci. Med. Rehabil. 2020;12(1):70. https://doi.org/10.1186/s13102-020-00219-3

Çetin S., Ece C., Paksoy M., Çetin H.N. Comparison of Lipid and Lipoprotein Values of Wrestlers and Soccer Players. Turk. J. Pharm. Sci. 2020;17(2):172–176. https://doi.org/10.4274/tjps.galenos.2018.66934

Walker A.J., McFadden B.A., Sanders D.J., Bozzini B.N. Early Season Hormonal and Biochemical Changes in Division I Field Hockey Players: Is Fitness Protective? J. Strength Cond. Res. 2020;34(4):975–981. https://doi.org/10.1519/JSC.0000000000003492

Nowakowska А., Kostrzewa-Nowak D., Buryta R., Nowak R. Blood Biomarkers of Recovery Efficiency in Soccer Players.Int. J. Environ. Res. Public Health. 2019;16(18):3279. https://doi.org/10.3390/ijerph16183279

Sellami M., Al-Muraikhy S., Al-Jaber H., Al-Amri H. Al-Mansoori L., A MazloumN., et al. Age and Sport IntensityDependent Changes in Cytokines and Telomere Length in Elite Athletes. Antioxidants (Basel). 202128;10(7):1035. https://doi.org/10.3390/antiox10071035

Sellami M., Al-Muraikhy S., Al-Jaber H., Al-Amri H. AlMansoori L., A MazloumN., et al. Age and Sport Intensity-Dependent Changes in Cytokines and Telomere Length in Elite Athletes. Antioxidants (Basel). 202128;10(7):1035. https://doi.org/10.3390/antiox10071035

Sohail M.U., Al-Mansoori L., Al-Jaber H., Georgakopoulos C., Donati F., BotrèF., et al. Assessment of Serum Cytokines and Oxidative Stress Markers in Elite Athletes Reveals Unique Profiles Associated With Different Sport Disciplines. Front. Physiol. 2020;11:600888. https://doi.org/10.3389/fphys.2020.600888

Sohail M.U., Al-Mansoori L., Al-Jaber H., Georgakopoulos C.,Donati F., BotrèF., et al. Assessment of Serum Cytokines and Oxidative Stress Markers in Elite Athletes Reveals Unique Profiles Associated With Different Sport Disciplines. Front. Physiol. 2020;11:600888. https://doi.org/10.3389/fphys.2020.600888

Kaier T.E., Twerenbold R., Puelacher C., Marjot J., Imambaccus N., Boeddinghaus J., et al. Direct Comparison of Cardiac Myosin-Binding Protein C With Cardiac Troponins for the Early Diagnosis of Acute Myocardial Infarction Circulation. 2017;136(16):1495–1508. https://doi.org/10.1161/CIRCULATIONAHA.117.028084

Kaier T.E., Twerenbold R., Puelacher C., Marjot J., Imambaccus N., BoeddinghausJ., et al. Direct Comparison of Cardiac Myosin-Binding Protein C With Cardiac Troponins for the Early Diagnosis of Acute Myocardial Infarction. Circulation. 2017;136(16):1495–1508. https://doi.org/10.1161/CIRCULATIONAHA.117.028084

Bordoni L., Petracci I., Pelikant-Malecka I., Radulska A., Piangerelli M., Samulak J.J., et al. Mitochondrial DNA copy number and trimethylamine levels in the blood: New insights on cardiovascular disease biomarkers. FASEB J. 2021;35(7):e21694. https://doi.org/10.1096/fj.202100056R

Osawa Y., Nomura K., Kimira Y., Kushibe S., Takeyama K-I., Nagao M., et al. Monitoring urinary collagen metabolite changes following collagen peptide ingestion and physical activity using ELISA with anti active collagen oligopeptide antibody. Sci. Rep. 2021;11(1):13527. https://doi.org/10.1038/s41598-021-92934-1.

Osawa Y.,Nomura K.,Kimira Y., Kushibe S., Takeyama K-I., Nagao M., et al. Monitoring urinary collagen metabolite changes following collagen peptide ingestion and physical activity using ELISA with anti active collagen oligopeptide antibody. Sci. Rep. 2021;11(1):13527. https://doi.org/10.1038/s41598-021-92934-1.

Radišić Biljak V., Đuras A., Čičak H., Vidranski V., Vidranski T., PeersmanN., et al. Women in sports: The applicability of reference intervals for 6 commercially available testosterone immunoassays. Clin. Biochem. 2020;84:55–62. https://doi.org/10.1016/j.clinbiochem.2020.06.006

La’ulu S.L., Kalp K.J., Straseski J.A. How low can you go? Analytical performance of five automated testosterone immunoassays. Clin. Biochem. 2018;58:64–71. https://doi.org/10.1016/j.clinbiochem.2018.05.008

Самушия К.А., Загородный Г.М. Проблемы и особенности медицинского обеспечения в общей системе подготовки спортсменов. Прикладная спортивная наука. 2019;(1(9)):87-94.

Samushia K.A., Zagharodny G.M. Problems and features of medical support in the general system of training athletes. Prikladnaya sportivnaya nauka [Applied sports science]. 2019;(1(9)):87–94 (In Russ.).

Arab L. Biomarkers of fat and fatty acid intake. J. Nutr. 2003;133(Suppl. 3):925S–932S. https://doi.org/10.1093/jn/133.3.925S

Ramos-Lopez O., Milagro F.I., Allayee H.,Chmurzynska A., Choi M.S., Curiet R., et al. Guide for Current Nutrigenetic, Nutrigenomic and Nutriepigenetic Approaches for Precision Nutrition Involving the Prevention and Management of Chronic Diseases Associated with Obesity. J. Nutrigenet. Nutrigenomics 2017;10(1-2):43–62. https://doi.org/10.1159/000477729

Felisbino K., Granzotti J.G., Bello-Santos L., Guiloski I.C. Nutrigenomics in Regulating the Expression of Genes Related to Type 2 Diabetes Mellitus. Front. Physiol. 2021;12:699220. https://doi.org/10.3389/fphys.2021.699220

De Vries J., Antoine J.M., Burzykowski T., Chiodini, A. Markers for nutrition studies: Review of criteria for the evaluation of markers. Eur. J. Nutr. 2013;52(7):1685–1699. https://doi.org/10.1007/s00394-013-0553-3

Picó C., Serra F., Rodríguez A.M., Keijer J., Palou A. Biomarkers of Nutrition and Health: New Tools for New Approaches. Nutrients. 2019 May 16;11(5):1092. https://doi.org/10.3390/nu11051092

Khaled K., Hundley V., Tsofliou F. Poor Dietary Quality and Patterns Are Associated with Higher Perceived Stress among Women of Reproductive Age in the UK. Nutrients. 2021;13(8):2588. https://doi.org/10.3390/nu13082588

Cornelis M.C., Byrne E.M., Esko T., Nalls M.A., Ganna A., Paynter N., et al. Genome-wide meta-analysis identifies six novel loci associated with habitual consumption. Mol. Psychiatry. 2015;20(5):647–656. https://doi.org/10.1038/mp.2014.107

Afman L., Milenkovic D., Roche H.M. Nutritional aspects of metabolic inflammation in relation to health–insights from transcriptomic biomarkers in PBMC of fatty acids and polyphenols. Mol. Nutr. Food Res. 2014:58(8):1708–1720. https://doi.org/10.1002/mnfr.201300559

Priego T., Sanchez J., Pico C., Ahrens W., De Henauw S., Kourides Y., et al. TAS1R3 and UCN2 Transcript Levels in Blood Cells Are Associatedwith Sugary and Fatty Food Consumption in Children. J. Clin. Endocrinol. Metab. 2015;100(9):3556–3564. https://doi.org/10.1210/JC.2015-1976

Ayoub H.M., McDonal M.R., Sullivan J.A., Tsao R., Meckling K.A. Proteomic Profiles of Adipose and Liver Tissues from an Animal Model of Metabolic Syndrome Fed Purple Vegetables. Nutrients. 2018;10(4):456. https://doi.org/10.3390/nu10040456

Ayoub H.M., McDonal, M.R., Sullivan J.A., Tsao R., Meckling K.A. Proteomic Profiles of Adipose and Liver Tissues from an Animal Model of Metabolic Syndrome Fed Purple Vegetables. Nutrients. 2018;10(4):456. https://doi.org/10.3390/nu10040456

Senechal S., Kussmann M. Nutriproteomics: Technologies and applications for identification and quantification of biomarkers and ingredients. Proc. Nutr. Soc. 2011;70(3):351–364. https://doi.org/10.1017/S0029665111000528

Marshall J., Bowden P., Schmi J.C. Creation of a federated database of blood proteins: Apowerful new tool for finding and characterizing biomarkers in serum. Clin. Proteomics. 2014;11(1):3. https://doi.org/10.1186/1559-0275-11-3

Mathers J.C. Nutrigenomics in the modern era. Proc. Nutr. Soc. 2017;76(3):265–275. https://doi.org/10.1017/S002966511600080X

Keijer J., van Helden Y.G., Bunschoten A., van Schothorst E.M. Transcriptome analysis in benefit-risk assessment of micronutrients and bioactive food components. Mol. Nutr. Food Res. 2010;54(2):240–248. https://doi.org/10.1002/mnfr.200900304

O’Gorman A., Brennan L. The role of metabolomics in determination of new die-tary biomarkers. Proc. Nutr. Soc. 2017;76(3):295–302. https://doi.org/10.1017/S0029665116002974

Van Duynhoven J.M., Jacobs D.M. Assessment of dietary exposure in humans: The role of NMR. Prog. Nucl. Magn. Reson. Spectrosc. 2016;96:58–72. https://doi.org/10.1016/j.pnmrs.2016.03.001

Guertin K.A., Moore S.C., Sampson J.N., Huang W.Y., Xiao Q., Stolzenberg-Solomon R.Z., et al. Metabolomics in nutritional epidemiology: Identifying metabolites associated with diet and quantifying their potential to uncover diet-disease relations in populations. Am. J. Clin. Nutr. 2014;100(1):208-217. https://doi.org/10.3945/ajcn.113.078758

Pena-Romero A.C., Navas-Carrillo D., Marin F., OrenesPinero E. The future of nutrition: Nutrigenomics and nutrigenetics in obesity and cardiovascular diseases. Crit. Rev. Food Sci. Nutr. 2018;58(17):3030–3041. https://doi.org/10.1080/10408398.2017.1349731

Day K.J., Adamski M.M., Dordevic A.L., Murgia C. Genetic Variations as Modifying Factors to Dietary Zinc Requirements-A Systematic Review. Nutrients. 2017;9(2):148. https://doi.org/10.3390/nu9020148

Papamichael M.M., Katsardis C., Tsoukalas D., Itsiopoulos C., Erbas B. Plasma lipid biomarkers in relation to BMI, lung function, and airway inflammation in pediatric asthma. Metabolomics. 2021;17(7):63. https://doi.org/10.1007/s11306-021-01811-5

Zhu S., Zhao X.E., Liu H. Recent advances in chemical derivatization-based chromatography-mass spectrometry methods for analysis of aldehyde biomarkers. Se Pu. 2021;39(8):845–854. https://doi.org/10.3724/SP.J.1123.2021.02023

Obstfeld A.E., Patel K., Boyd J.C., Drees J., Holmes D.T., et al. Data Mining Ap-proaches to Reference Interval Studies. Clin. Chem. 2021;67(9):1175–1181. https://doi.org/10.1093/clinchem/hvab137

Coisnon C., Mitchell M.A., Rannou B., Le Boedec K. Subjective assessment of frequency distribu-tion histograms and consequences on reference interval accuracy for small sample sizes: A computer-simulated study. Vet Clin Pathol. 2021;50(3):427–441. https://doi.org/10.1111/vcp.13000

Harb S.C., Bhat P., Cremer P.C., Wu Y., Cremer L.J., Berger S., et al. Prognostic Value of Functional Capacity in Different Exercise Protocols. J. Am. Heart Assoc. 2020;9(13):e015986. https://doi.org/10.1161/JAHA.119.015986

Ruzhanskaya A., Ichihara K., Evgina S., Skibo I., Vybornova N., Vasiliev A., et al. Sources of variation and establishment of Russian reference intervals for major hormones and tumor markers. PLOS ONE. 2021;16(1):e0234284. https://doi.org/10.1371/journal.pone.0234284

Al-Mallah M.H., Juraschek S.P., Whelton S., Dardari Z.A., Ehrman J.K., Michos E.D., et al. Sex Differences in Cardiorespiratory Fitness and All-Cause Mortality: The Henry Ford ExercIse Testing (FIT) Project. Mayo Clin. Proc. 2016;91(6):755–762. https://doi.org/10.1016/j.mayocp.2016.04.002

da Silva B.V.C., Simim M.A.M., da Silva R.B., Mendes E.L., Ide B.N., Marocolo M., et al. Time Course of Recovery for Performance Attributes and Circulating Markers of Muscle Damage Following a Rugby Union Match in Amateur Athletes. Sports (Basel). 2020;8(5):64. https://doi.org/10.3390/sports8050064

Daly L.S., Catháin C.Ó., Kelly D.T. Gaelic Football MatchPlay: Performance Attenuation and Timeline of Recovery. Sports (Basel). 2020;8(12):166. https://doi.org/10.3390/sports8120166

de Carvalho G., Girasol C.E., Gonçalves L.G.C., Guirro E.C., de Jesus Guirro R.R. Correlation between skin temperature in the lower limbs and biochemical marker, performance data, and clinical recovery scales. PLoS One. 2021;16(3):e0248653. https://doi.org/10.1371/journal.pone.0248653

Huggins R.A., Fortunati A.R., Curtis R.M., Looney D.P., West C.A., Lee E.C., et al. Monitoring Blood Biomarkers and Training Load Throughout a Collegiate Soccer Season. J. Strength Cond. Res. 2019;33(11):3065–3077. https://doi.org/10.1519/JSC.0000000000002622

Загородный Г.М. О совершенствовании научно-методического и медицинского обеспечения подготовки спортсменов национальных и сборных команд Республики Беларусь. Прикладная спортивная наука. 2018;(1(7)):92–98.

Zaharodny G.M. About improving the scientific, methodological and medical support of training athletes of national and national teams of the Republic of Belarus. Prikladnaya sportivnaya nauka [Applied sports science]. 2018;(1(7)):92–98 (In Russ.).

Ачкасов Е.Е., Машковский Е.В., Безуглов Э.Н., Юрку К.В. Медико-биологические аспекты восстановления в профессиональном и любительском спорте. Медицинский вестник Северного Кавказа. 2018;13(1–1):126–132.

Achkasov E.E., Mashkovsky E.V., Bezuglov E.N., Yurku K.V. Medical and biological aspects of recovery in professional and amateur sports. Meditsinskii vestnik Severnogo Kavkaza = Medical News of the North Caucasus. 2018;13(1–1):126–132 (In Russ.).

The authors declare that there are no conflicts of interest present.