Influence of ATACL compounds on some biochemical indicators in conditions of exhausting exercise in animals

Abstract

Objective: to study the effect of the ATACL (4-hydroxy-3,5-di-tert-butylcinnamicacid) compound on some biochemical parameters under conditions of exhausting exercise in animals. Materials and methods: the study was conducted to study the effect of a cinnamic acid derivative on certain biochemical parameters under conditions of exhausting exercise in animals. The experiment was performed on 40 outbred male mice weighing 22-24 grams. Animals were subject to daily exhausting exercise for 5 days in the treadmill running test. The test compound ATACL was administered intragastrically at a dose of 100 mg/kg for 30 minutes before the exercise. At the end of the experiment, the animals were decapitated and blood was taken to assess lactic, pyruvic acid, the lactate/pyruvate coefficient and myoglobin as the marker of muscle damage. Results: It was found that upon exercise, 4-hydroxy-3,5-di-tert-butylcinnamic acid neutralizes the acidic pH. 10.7 times (p<0.05) increase in the concentration of pyruvate in the blood was observed compared to the negative control group, and a decrease in the lactate / pyruvate coefficient by 28.6 times (p<0.05). At the same time, the level of myoglobin in the group receiving ATACL was 2.1 times lower compared to the negative control group (p<0.05). No significant differences were observed in terms of lactate, pyruvate, and myoglobin concentration in the group treated with ATACL, in comparison with the group of animals treated with Metaprot. Conclusions: the data obtained allow us to recommend this compound as a corrector of biochemical shifts that are possible during physical exertion.