Some indicators of free radical oxidation and antioxidant protection in the erythrocytes of former athletes
https://doi.org/10.47529/2223-2524.2021.3.5
Abstract
Objective: to study the main indicators of free radical oxidation and antioxidant protection in the erythrocytes of former athletes, depending on the period of termination of sports.
Materials and methods: 24 former male athletes aged 19–29 years were examined, who were divided into 2 groups of 12 people each (1st group — former athletes who stopped training for up to 2 years; 2nd — over 2 years). The control group consisted of 15 practically healthy untrained student volunteers of the same age. In erythrocytes, spectrophotometrically (spectrophotometer Shimadzu 1240, Japan), the activity of antioxidant enzymes was determined: superoxide dismutase (SOD) (K.F. 1.15.1.1) — by inhibiting the reduction of nitro blue tetrazolium by superoxide anion radical at λ = 540 nm, after preliminary processing of erythrocytes by the method of Dubinina E.E. and others [8]; catalase (K.F. 1.11.1.6) — according to the rate of utilization of hydrogen peroxide at λ = 260 nm; glutathione peroxidase (HP) (KF 1.11.1.9) — by the change in the content of reduced glutathione in samples before and after incubation of the substrate with dithiobis-nitrobenzoic acid at λ = 412 nm; glutathione reductase (GR) (K.F. 1.6.4.2) — according to the catalytic NADPH ∙ H+-dependent transformation of the oxidized form of glutathione into the reduced form, the intensity of which was estimated by the rate of decrease in the extinction of samples at λ = 340 nm, at which the NADPH H+ solution has a maximum light absorption (Warburg test).
Results: the direction of the shifts in the state of the oxidative balance was established depending on the period of detraining. A significantly lower value of the total antioxidant activity was revealed in the former athletes of the 1st group. This phenomenon is probably associated with a decrease in the efficiency of the enzymatic link of the antioxidant defense in the absence of regular physical exertion, and, as a consequence, an increased consumption of non-enzymatic antioxidants. It was found that the early post-sports period is characterized by significantly lower, in comparison with the control group, the values of the activity of the first-order enzymes (superoxide dismutase) against the background of the increased activity of the second-order enzymes (catalase).
Conclusions: the obtained data can be recommended for monitoring the state of athletes who stopped playing sports and taken into account when prescribing rehabilitation measures for the corresponding contingent.
About the Author
A. V. ElikovRussian Federation
Anton V. Elikov, M.D., Ph.D. (Medicine), Associate Professor of the Department of Chemistry
112, K. Marks str., Kirov, 610027
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Review
For citations:
Elikov A.V. Some indicators of free radical oxidation and antioxidant protection in the erythrocytes of former athletes. Sports medicine: research and practice. 2021;11(3):12-17. (In Russ.) https://doi.org/10.47529/2223-2524.2021.3.5